Maltose Binding Protein antibody

Catalog No. ABIN3020578

This Mouse Monoclonal antibody specifically detects Maltose Binding Protein in WB. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Maltose Binding Protein antibody (ABIN3020578)

Target: Maltose Binding Protein (MBP)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Maltose Binding Protein antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Maltose Binding Protein Antibody

Purification: Affinity purification

Immunogen: An MBP-Tag fusion protein

Isotype: IgG

Application Details

Application Notes: WB: 1:1000 1:3000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C / -80 °C

References for anti-Maltose Binding Protein antibody (ABIN3020578)

Xin, Zhang, Zhang, Li, Wang, Wang, Wang, Qiu, Song: "CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules." in: Developmental and comparative immunology, Vol. 53, Issue 2, pp. 339-48, (2016) (PubMed).

Target Details for Maltose Binding Protein

Target: Maltose Binding Protein (MBP)

Alternative Name: Maltose Binding Protein

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (myelin basic protein) TAG in some expression systems.